质粒名称: | pLCN DSB Repair Reporter (DRR) |
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出品公司: | 爱迪基因 |
目录编号: | 98895 |
存储实验室: | Jan Karlseder |
载体骨架基本信息 |
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载体名称: | pLenti CMV Neo |
载体出品公司: | Eric Campeau |
载体抗性: | 氨苄青霉素 |
空载体大小: | 6700 bp |
完整质粒大小: | 8360 bp |
载体类型: | 慢病毒载体 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Neomycin-T2A-ISce1-ISce1-eGFP |
物种来源: | Synthetic |
插入大小: | 1660 bp |
启动子 | CMV |
5' 测序引物 | CGCAAATGGGCGGTAGGCGTG |
文献引用方法 |
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材料和方法部分: | pLCN DSB Repair Reporter (DRR) was a gift from Jan Karlseder (Addgene plasmid # 98895 ; http://n2t.net/addgene:98895 ; RRID:Addgene_98895) |
参考文献部分: | Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN. Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J. Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023. 10.1038/nature24023 PubMed 28959974 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLCN DSB Repair Reporter (DRR) 8444 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.. ACCESSION . VERSION . KEYWORDS pLCN DSB Repair Reporter (DRR) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8444) AUTHORS Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J TITLE Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN. JOURNAL Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023. PUBMED 28959974 REFERENCE 2 (bases 1 to 8444) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8444 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..22 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 37..67 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 75..91 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 80..102 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 99..115 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 99..115 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 134..154 /label=T3 /note="T3 promoter, forward primer" promoter 136..154 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 182..408 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 409..589 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 636..761 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1254..1487 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1672..1716 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1983..2099 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2122..2425 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2426..2629 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2580..2600 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2626..2650 /label=LNCX /note="Human CMV promoter, forward primer" CDS 2687..3487 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2750..2769) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3360..3379 /label=Neo-F /note="Neomycin resistance gene, forward primer" CDS 3488..3541 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 3584..3610 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3630..4346 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" misc_feature 4458..5046 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4511..4531) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4929..4940) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5144..5377 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 5449..5570 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5486..5505) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5540..5559 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 5610..5745 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5672..5691 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(5765..5784) /label=T7 /note="T7 promoter, forward primer" promoter complement(5766..5784) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5794..5811) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5794..5810) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5803..5825) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 5952..6407 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6039..6058) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6249..6270 /label=F1ori-F /note="F1 origin, forward primer" promoter 6433..6537 /gene="bla" /label=AmpR promoter CDS 6538..7398 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6756..6775) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7569..8157 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8058..8077 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8311..8328 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta 181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta 1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 2101 tcgataagct tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 2161 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 2221 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 2281 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 2341 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 2401 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 2461 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 2521 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 2581 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa 2641 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccacatgg gatcggccat 2701 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 2761 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 2821 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 2881 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 2941 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 3001 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 3061 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 3121 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 3181 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 3241 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 3301 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 3361 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 3421 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 3481 gttcttcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atccgggccc 3541 cattagggat aacagggtaa tggcggcggc ggatccggta tgatacccat acgatgttcc 3601 tgactatgcg attaccctgt tatccctaca tggtgagcaa gggcgaagaa ctctttacag 3661 gcgtcgttcc catcttggtt gagcttgacg gggacgtcaa cggccataaa ttcagcgttt 3721 ccggggaggg agagggcgat gcaacatacg ggaagctgac tttgaagttt atctgtacga 3781 ctggaaaact gccggtgcct tggccgacgc tggtgactac tctgacatat ggtgtgcagt 3841 gtttttccag atatcctgat catatgaagc agcatgattt cttcaagagc gcgatgccag 3901 agggctacgt gcaggaaaga acgatcttct tcaaagacga tggtaattac aaaactcggg 3961 cagaggtcaa attcgaggga gatacgctgg taaaccggat cgagctgaag ggcatcgatt 4021 ttaaggaaga tggtaacatc ctgggacaca aactggagta taattataac tcccataatg 4081 tctatattat ggctgacaag cagaagaacg ggatcaaggt gaactttaag atccggcaca 4141 atatcgaaga tggctccgtt cagctggccg atcattatca acaaaacaca cctattggag 4201 atgggccagt cctgttgcca gataatcact atctgtccac acagtccgcc ttgtccaaag 4261 acccgaacga gaagagagac cacatggtgc tgctggagtt tgttacggcg gccgggatca 4321 cgttgggcat ggatgagctt tacaagtgaa gcggccgcga ctcataatca gccataccac 4381 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 4441 taaaatgaat gcaattgaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 4501 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 4561 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 4621 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 4681 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 4741 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 4801 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 4861 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 4921 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 4981 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 5041 ccccgcttgt tgttaggcgt cgacagtact aagctttctt taagaccaat gacttacaag 5101 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac 5161 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 5221 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 5281 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 5341 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 5401 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 5461 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 5521 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 5581 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 5641 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 5701 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa 5761 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 5821 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 5881 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 5941 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 6001 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 6061 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 6121 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 6181 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 6241 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 6301 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 6361 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 6421 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 6481 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 6541 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 6601 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 6661 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 6721 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 6781 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 6841 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 6901 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 6961 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 7021 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 7081 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 7141 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 7201 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 7261 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 7321 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 7381 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 7441 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 7501 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 7561 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 7621 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 7681 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 7741 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 7801 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 7861 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 7921 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 7981 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 8041 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 8101 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 8161 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 8221 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 8281 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 8341 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 8401 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caat //
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